fibroblast growth basal medium-2 with fgmtm-2 singlequotstm supplements (cc-4126)  (Lonza)

Journal: Scientific Reports

Article Title: Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells

doi: 10.1038/s41598-020-80484-x

Figure Lengend Snippet: In vitro anti-cell proliferation analysis of MGDG extracted from Synechocystis sp. PCC 6803 (solid line) and MGDG standard (dotted line) in ( a ) BT-474 and ( b ) MDA-MB-231 cell lines. Mean percentage values are presented from six replicates (n = 6) for each drug dosage. The MGDG from the Synechocystis sp. inhibits up to 70% ( p < 0.001) and 60% ( p < 0.001) of BT-474 and MDA-MB-231 cancer cells, respectively where MGDG standard does not show any toxicity within the concentration range of 0–200 ng/ml. *** indicates the p value < 0.001 showing a statistically significant difference between % cell death in MGDG extracted from the Synechocystis sp. and MGDG standard. ( c ) MGDG extracted from the Synechocystis sp. does not show any cytotoxic side effects in human dermal fibroblasts normal cell line control.

Article Snippet: BT-474, MDA-MB-231, and human dermal fibroblast cells (ATCC) were cultured in Hybri-care ATCC 46-X medium, RPMI 1640, and fibroblast growth factor supplemented basal medium (ATCC), respectively, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C and 5% CO .

Techniques: In Vitro, Concentration Assay, Control

Journal: Viruses

Article Title: Favipiravir and Ribavirin Inhibit Replication of Asian and African Strains of Zika Virus in Different Cell Models

doi: 10.3390/v10020072

Figure Lengend Snippet: Zika virus (ZIKV) replication efficiency in favipiravir and ribavirin-treated human dermal fibroblasts (HDFs) and A549 cells. ( A ) HDFs, A549, and Vero cells were treated with various doses of favipiravir (μM), or ribavirin (μg/mL) and incubated for 24 h. At the end of incubation, cell viability was determined using MTT assays. Each value represents the mean ± SEM ( n = 4). ( B ) HDFs and A549 cells were infected with ZIKV at MOI of 1 for indicated times. ZIKV E and NS5 expressions are shown. * p < 0.05; ** p < 0.01; *** p < 0.001, compared with mock control. ( C ) HDFs and ( D ) A549 cells were infected with ZIKV at MOI of 1 and treated with either a DMSO control (0 μM), favipiravir (1, 10, 25, and 50 μM), or ribavirin (1, 10, 25, and 50 μg/mL) for 24 h. qRT-PCR was performed to measure ZIKV E and NS5 mRNA levels. The expression of viral transcripts was calculated in relation to the expression level of GAPDH mRNA and expressed as fold-changes relative to expression levels in DMSO control-treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001, compared with DMSO control-treated cells.

Article Snippet: Human dermal fibroblasts (HDFs) (Lonza, Basel, Switzerland) were grown as adherent cultures in fibroblast basal medium supplemented with fibroblast growth media (FGM) SingleQuots (Lonza) and cultured as described previously [ ].

Techniques: Virus, Incubation, Infection, Control, Quantitative RT-PCR, Expressing